Polyclonal antibody production involves immunising an animal with a specific antigen, triggering the immune system to produce antibodies targeting different epitopes. These antibodies are harvested from serum, providing broad binding specificity.
Polyclonal antibodies recognise multiple epitopes on the target antigen, providing broader binding specificity compared to monoclonals.
Higher sensitivity in assays enabling detection of low-abundance targets due to binding multiple epitopes.
Generated relatively quickly and cost-effectively compared to other antibody production methods.
Useful for detecting various forms and isoforms of target proteins across applications.
Mimics natural immune response, providing diverse antibody repertoire similar to in vivo.
May exhibit cross-reactivity with related antigens for broader detection.
We prepare the immunogen using your provided antigen or our peptide synthesis service.
Animals are immunised with the prepared antigen following our optimized protocols.
Regular titer testing to monitor antibody response and determine optimal bleed time.
Affinity or Protein A/G purification to isolate high-quality antibodies.
Western blot validation with the target antigen to ensure quality.
Contact our team to discuss your custom polyclonal antibody requirements.
Request a QuoteWe offer polyclonal antibody production in multiple species including rabbit, goat, rat, mouse, and sheep. Each species has different advantages depending on your application needs.
Typically 1-2mg of purified antigen is sufficient for a standard immunisation protocol. For peptide antigens, we offer in-house peptide synthesis services.
All antibodies undergo rigorous QC including ELISA titer testing, Western blot validation, and purity assessment by SDS-PAGE. We guarantee a minimum titer of 1:10,000.
Yes, we offer multiple bleed schedules to maximize yield from a single immunisation. Contact us to discuss your specific requirements.