Applications & Protocols

Step-by-step experimental protocols for various assays and applications.

Detection and analysis of proteins using SDS-PAGE and immunoblotting.

1 Prepare protein samples

2 Run SDS-PAGE gel

3 Transfer to membrane

4 Probe with primary antibody

Detection of antigens in tissue sections using specific antibodies.

1 Deparaffinize and rehydrate

2 Antigen retrieval

3 Block endogenous peroxidase

4 Incubate with primary antibody

Quantitative detection of antigens or antibodies in samples.

1 Coat plate with capture antibody

2 Add sample and incubate

3 Add detection antibody

4 Add substrate and read

Visualization of target proteins in cells using fluorescent tags.

1 Fix and permeabilize cells

2 Block non-specific binding

3 Stain with primary/secondary

4 Mount and image