ELISA Kits
Lablisa® MF-Human IL-6(Interleukin 6) ELISA Kit
Catalog No.: 1PLOHF225
| Sensitivity | 2 |
| Detection Range | 3 |
| Assay Duration | 5 |
| Reactivity | 4 |
Description
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human IL-6. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.
Technical Specifications
| Catalog Number | 1PLOHF225 |
| Sensitivity | 2 |
| Detection Range | 3 |
| Assay Duration | 5 |
| Reactivity | 4 |
Product Overview
| Product name: | Lablisa® MF-Human IL-6(Interleukin 6) ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor , HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A |
| Assay Type: | Sandwich |
| Sensitivity: | 0.47 pg/mL |
| Standard: | 50pg/mL |
| Range: | 0.78-50pg/mL |
| Sample Type: | Serum, Plasma and Tissue homogenate and Other biological samples；Sample volume：25μL |
| Assay Length: | 3.5h |
| Research Area: | Cardiovascular, Cell biology, Neuroscience, Cancer, Immunology, Metabolism |
Standard curve
| Concentration (ng/mL) | OD | Corrected OD |
|---|---|---|
| 100.00 | 2.366 | 2.311 |
| 50.00 | 1.564 | 1.509 |
| 25.00 | 0.961 | 0.906 |
| 12.50 | 0.529 | 0.474 |
| 6.250 | 0.274 | 0.219 |
| 3.130 | 0.159 | 0.104 |
| 1.570 | 0.098 | 0.043 |
| 0.00 | 0.055 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level were tested on 3 different plates, 20 replicates in each plate, respectively.
Recovery
The recovery of spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=8) | 82-95% | 88% |
| EDTA plasma(n=8) | 95-107% | 101% |
| Cell culture media (n=8) | 94-107% | 100% |
Linearity
Samples were spiked with high concentrations of target proteins and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 93-102% | 81-96% | 93-106% | 82-95% |
| EDTA plasma(n=5) | 92-101% | 87-98% | 85-92% | 80-96% |
| Cell culture media (n=5) | 86-96% | 93-104% | 89-97% | 85-94% |
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Protocol
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody and biotinylated detection antibody specific for Human IL-6. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Human IL-6. You can calculate the concentration of Human IL-6 in the samples by comparing the OD of the samples to the standard curve.
