Antibodies
Bax Recombinant Rabbit Monoclonal Antibody
Catalog No.: LRC-orb608054
Recombinant rabbit monoclonal antibody recognizing human, mouse, and rat Bax protein. Validated for multiple applications.
Host Species
Rabbit
Clonality
Monoclonal
Immunogen
Synthetic peptide corresponding to human Bax
Conjugation
Unconjugated
Applications
WB, IHC-P, IHC-Fr, IF, FC
Reactivity
Human, Mouse, Rat
Dilution Range
WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test
Storage
-20°C for 12 months
Product Overview
Bax is a key pro-apoptotic member of the BCL-2 family proteins. It plays a crucial role in regulating mitochondrial outer membrane permeabilization (MOMP) and initiating the intrinsic apoptosis pathway. Upon apoptotic stimuli, Bax translocates from the cytosol to mitochondria, where it oligomerizes and forms pores, leading to the release of cytochrome c.
Full Description
Bax (BCL2-associated X protein) is a pro-apoptotic member of the BCL-2 family. This recombinant rabbit monoclonal antibody recognizes human, mouse, and rat Bax protein. It is suitable for Western Blot, Immunohistochemistry, Immunofluorescence, and Flow Cytometry applications.
Research Area
Apoptosis research, cancer research, cell cycle studies, mitochondrial biology
Applications
| Application | Dilution | Species |
|---|---|---|
| WB | WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test | Human, Mouse, Rat |
| IHC-P | WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test | Human, Mouse, Rat |
| IHC-Fr | WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test | Human, Mouse, Rat |
| IF | WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test | Human, Mouse, Rat |
| FC | WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test | Human, Mouse, Rat |
Technical Specifications
| Catalog Number | LRC-orb608054 |
| Product Name | Bax Recombinant Rabbit Monoclonal Antibody |
| Host Species | Rabbit |
| Clonality | Monoclonal |
| Clone Number | BLR010F |
| Immunogen | Synthetic peptide corresponding to human Bax |
| Species Reactivity | Human, Mouse, Rat |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Affinity purification |
| Form | Liquid |
| Storage Buffer | PBS, pH 7.4, 50% glycerol, 0.02% sodium azide |
| Storage Temperature | -20°C for 12 months |
| Concentration | 1 mg/mL |
| Tested Applications | WB, IHC-P, IHC-Fr, IF, FC |
| Recommended Dilution | WB: 1:1000-2000, IHC-P: 1:100-500, IF: 1:100-500, FC: 1μg/Test |
Protocol
Western Blot Protocol:
1. Load 20-30 μg protein per lane
2. Transfer to PVDF membrane
3. Block with 5% non-fat milk in TBST, 1hr
4. Incubate primary antibody 1:1000 overnight at 4°C
5. Wash 3x with TBST
6. Incubate HRP-secondary 1:5000, 1hr
7. Wash and detect with ECL
1. Load 20-30 μg protein per lane
2. Transfer to PVDF membrane
3. Block with 5% non-fat milk in TBST, 1hr
4. Incubate primary antibody 1:1000 overnight at 4°C
5. Wash 3x with TBST
6. Incubate HRP-secondary 1:5000, 1hr
7. Wash and detect with ECL
