Antibodies
Anti-GAPDH Rabbit Polyclonal Antibody
Catalog No.: LRC-ORB10805
Validated rabbit polyclonal antibody against human GAPDH. Ideal loading control for Western blot applications.
Host Species
Rabbit
Clonality
Polyclonal
Immunogen
Full length human GAPDH recombinant protein
Conjugation
Unconjugated
Applications
WB, IHC-P, IF, FC
Reactivity
Human, Mouse, Rat, Monkey
Dilution Range
WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500
Storage
-20°C for 12 months
Product Overview
GAPDH is a 36 kDa enzyme that catalyzes the oxidative phosphorylation of glyceraldehyde-3-phosphate. It is widely used as a housekeeping gene and protein loading control due to its stable expression across most tissue types and experimental conditions.
Full Description
GAPDH (glyceraldehyde-3-phosphate dehydrogenase) is a key enzyme in glycolysis. It is constitutively expressed at high levels in most tissues and is commonly used as a loading control for Western blotting. This antibody recognizes GAPDH protein in human, mouse, rat, and monkey samples.
Research Area
Cancer research, metabolism studies, cell biology, Western blot normalization
Applications
| Application | Dilution | Species |
|---|---|---|
| WB | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
| IHC-P | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
| IF | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
| FC | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 | Human, Mouse, Rat, Monkey |
Technical Specifications
| Catalog Number | LRC-ORB10805 |
| Product Name | Anti-GAPDH Rabbit Polyclonal Antibody |
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Full length human GAPDH recombinant protein |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Conjugation | Unconjugated |
| Isotype | IgG |
| Purification | Affinity purification |
| Form | Liquid |
| Storage Buffer | PBS, pH 7.4, 50% glycerol, 0.02% sodium azide |
| Storage Temperature | -20°C for 12 months |
| Concentration | 1 mg/mL |
| Tested Applications | WB, IHC-P, IF, FC |
| Recommended Dilution | WB: 1:1000-5000, IHC-P: 1:100-500, IF: 1:100-500 |
Protocol
Western Blot Protocol:
1. Load 20-30 μg protein per lane
2. Transfer to PVDF membrane
3. Block with 5% non-fat milk in TBST, 1hr
4. Incubate primary antibody 1:5000 overnight at 4°C
5. Wash 3x with TBST
6. Incubate HRP-secondary 1:5000, 1hr
7. Wash and detect with ECL
1. Load 20-30 μg protein per lane
2. Transfer to PVDF membrane
3. Block with 5% non-fat milk in TBST, 1hr
4. Incubate primary antibody 1:5000 overnight at 4°C
5. Wash 3x with TBST
6. Incubate HRP-secondary 1:5000, 1hr
7. Wash and detect with ECL
